Composite

Part:BBa_K307100:Design

Designed by: Joo-han Gwak   Group: iGEM10_CBNU-Korea   (2010-10-27)

rctA-ori2vc-rctB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 301
    Illegal BglII site found at 2130
    Illegal BglII site found at 2320
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1549
    Illegal SapI.rc site found at 2087


Design Notes

this DNA is vibrio cholerae chromosomeII replicaiton origin. It includes rctA and rctB, initiator and regulator protein.

Source

vibrio cholerae O1 biovar eltor str. N16961 chromosome II

References

venkova-canova et al.,"transcriptional inactivation of a regulatory site for replication of Vibrio cholerae chromosomeII",PNAS,Aug8.2006,vol.103,no.32,12051-12056

Elizabeth S.Egan et al.,"Autorepression of RctB, an Initiator of Vibrio cholerae ChromosomeII" Replication,Journal of bacteriology,jan.2006,vol.188,no.2,p.789-793

Debasish Pal, Venkova-canova et al.,"Multipartite Regulation of rctB, the Replication Initiator gene of Vibrio cholerae chromosomeII",Journal of bacteriology,nov.2005,vol.187,no.21,p.7167-7175

stephane Duigou et al.,"Independent control of replication initiation of the two vibrio cholerae chromosomes by DnaA and RctB",journal of bacteriology,sept.2006,vol.188,no.17,p.6419-6424

Anders Lobner-Olesen, "the DNA binding properties of RctB and Replication in vibrio cholerae",Rosklide University Department of Sciencem Systems and Models Microbiology Module2 spring 2007.